In wastewater treatment and environmental monitoring, sludge concentration (mixed liquor suspended solids, MLSS) is a key parameter. Excessively high sludge concentration can lead to oxygen deficiency, impaired microbial activity, and even sludge bulking.
To maintain stable operation, accurate and timely detection is essential. Two widely used methods are the gravimetric method (MLSS) and the sludge settling velocity method (SV30).
Gravimetric Method (MLSS)
The gravimetric method is the absolute reference technique for sludge concentration measurement. A well‑mixed sample of known volume is filtered through a pre‑weighed glass fiber filter. The filter with retained solids is dried at 103–105 °C to constant weight, then cooled and reweighed.
The difference in weight divided by the sample volume gives the MLSS value in mg/L or g/L. This method is highly accurate and serves as the benchmark for calibrating online sensors or other rapid tests. Its main drawback is the long turnaround time (several hours), which limits its use for real‑time process control.
Sludge Settling Velocity Method (SV30)
SV30 is the most common on‑site rapid test in activated sludge plants. A one‑liter graduated cylinder is filled with mixed liquor taken from the aeration tank and allowed to settle for 30 minutes. The volume of settled sludge (in mL) divided by 1000 mL (multiplied by 100 %) gives the SV30 value. Although SV30 does not directly yield MLSS, it provides a quick indication of sludge settleability and thickening performance.
In practice, the 30‑minute settling pattern also reveals important information: fast, compact settling indicates healthy sludge; slow or poor compaction suggests possible filamentous bulking. By combining SV30 with MLSS (from the gravimetric method), the sludge volume index (SVI) can be calculated, offering a comprehensive assessment of sludge quality.
Comparison and Practical Use
The gravimetric method delivers absolute, traceable data but is slow and requires laboratory equipment. SV30 is fast, simple, and provides immediate insight into settling behavior, yet it lacks absolute concentration values.
In routine operation, plant operators use SV30 several times per day to quickly detect trends or upsets, while the gravimetric method is performed periodically (e.g., once per shift or once per day) to calibrate and obtain precise MLSS numbers. The two methods complement each other, ensuring both rapid response and reliable control of high sludge concentration problems.

