Summer heat waves impose significant stress on activated sludge systems in wastewater treatment plants. Elevated water temperatures, often exceeding 35°C, directly influence mixed liquor suspended solids (MLSS) concentration through multiple biological and physical mechanisms. Understanding these effects is essential for operators to prevent process deterioration and effluent non‑compliance.
Microbial Metabolism Shifts
Most heterotrophic and nitrifying bacteria in activated sludge thrive in the mesophilic range of 20–35°C. When water temperature rises above 38°C, enzymatic activities begin to decline. Initially, metabolic rates accelerate, causing a temporary increase in substrate consumption and a modest rise in sludge growth. However, prolonged heat damages cell membranes and key enzyme systems, leading to microbial die‑off and autolysis.
As a result, the measured sludge concentration often drops after a short period of elevation. Nitrifiers are particularly vulnerable, and their inhibition may cause free ammonia accumulation, further suppressing the entire microbial community.
Settling Performance Deterioration
High temperatures reduce dissolved oxygen saturation. If aeration intensity is not adjusted accordingly, oxygen deficiency develops inside sludge flocs, promoting filamentous bacteria overgrowth. This condition triggers sludge bulking, characterized by loose, poorly settling flocs. Consequently, the sludge volume index rises, and the effective concentration in the return sludge becomes uneven, with significant loss of biomass over the final clarifier weirs.
Simultaneously, excessive extracellular polymer secretion may increase sludge viscosity, slowing sedimentation and raising sludge blanket levels. Even if the aeration tank MLSS value remains numerically stable, the separation efficiency deteriorates, leading to higher suspended solids in the effluent.
Measurement Interferences
Hot weather complicates accurate sludge concentration determination. Gravimetric methods and online optical or ultrasonic sensors require representative, homogeneous samples. High microbial activity in warm samples can continue enzymatic degradation of organic particles after sampling if no immediate cooling or fixation is applied, causing underestimation of true MLSS. Moreover, biofilms develop rapidly on sensor probes under summer conditions; without increased cleaning frequency, probe drift or delayed response may occur. Operators should therefore implement stricter sample preservation protocols and more frequent sensor maintenance during heat waves.
Operational Adjustments
To mitigate heat‑induced problems, process control must adapt. Endogenous respiration accelerates with temperature, meaning that maintaining the same MLSS requires more substrate. If influent organic load does not increase proportionally, reducing sludge concentration by 10–20% below normal seasonal targets is advisable to avoid excessive starvation and floc disintegration.
Additionally, oxygen transfer efficiency decreases at higher temperatures; excessive sludge concentration would worsen the oxygen deficit. Aeration should be increased or MLSS lowered accordingly. Wasting strategies should target old, dead biomass to prevent accumulation of inactive solids.

