Ammonia nitrogen (NH₃-N) enters water bodies from both natural and human activities
you generally cannot directly see ammonia nitrogen in water by its color alone.
Here's why and what methods do use color:
Ammonia is Colorless: Both free ammonia (NH?) and the ammonium ion (NH??) are colorless in water. Water appearing blue, green, brown, etc., is usually due to other factors like algae, dissolved organic matter, sediment, or tannins, not directly caused by ammonia.
Colorimetric Testing is Used: To measure ammonia nitrogen, scientists use chemical test kits or lab instruments. These methods rely on adding specific reagents that react with ammonia/ammonium to produce a visible color change:
Common Reagents: Salicylate-hypochlorite or Nessler's reagent.
Color Development: These reactions typically produce shades of yellow, green, or brown.
Intensity = Concentration: The intensity or depth of this developed color is directly proportional to the ammonia nitrogen concentration in the sample. This intensity is measured either:
Visually: By comparing the sample color to a standard color chart provided with the test kit.
Instrumentally: Using a spectrophotometer to measure light absorbance at a specific wavelength.
In summary: You cannot look at plain water and judge ammonia levels by its natural color. Instead, specific chemical tests create a measurable color change whose intensity indicates if ammonia nitrogen is present and whether it exceeds safe limits. Always follow the test kit instructions precisely and confirm critical results with professional labs if needed. Avoid older Nessler's reagent kits containing mercury due to toxicity.